Commercial Serologic Tests for Diagnosing Zika Virus Commercial Serologic Tests for Diagnosing Zika Virus

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This article examines the sensitivity and specificity of two tests for the serologic diagnosis of Zika virus infection. How do they stack up?Emerging Infectious Diseases

Source: Medscape Allergy HeadlinesCategory: Allergy & Immunology Tags: Infectious Diseases Journal Article Source Type: news

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Abstract
Japanese encephalitis virus (JEV) and West Nile virus (WNV) are two major mosquito borne flaviviruses belonging to same serocomplex. JEV is transmitted by Culex mosquitoes and the reservoir host for the virus is pigs and/or water birds. WNV is also transmitted by Culex mosquitoes and reservoir host in this case is birds. It can also be transmitted through contact with other infected animals, their blood, or other tissues. The envelope protein of these viruses is the major source of epitopes and provides protective immunity. Bioinformatics tools were used to identify conserved epitopes in the envelope prot…

New UCLA-led research suggests that 32% of children up to the age of 3 years who were exposed to the Zika virus during the mother ’s pregnancy had below-average neurological development.The study also found that fewer than 4% of 216 children evaluated had microcephaly —a smaller-than-normal head that is one of the hallmarks of the mosquito-borne disease. The heads of two of those children grew to normal size over time, the researchers reported.The studywas published in the journal Nature Medicine.The findings, conducted by UCLA researchers with colleagues in Rio de Janeiro, Brazil, where the disease was first d…

Publication date: Available online 28 June 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Parvez Singh Slathia, Preeti SharmaAbstractJapanese encephalitis virus (JEV) and West Nile virus (WNV) are two major mosquito borne flaviviruses belonging to same serocomplex. JEV is transmitted by Culex mosquitoes and the reservoir host for the virus is pigs and/or water birds. WNV is also transmitted by Culex mosquitoes and reservoir host in this case is birds. It can also be transmitted through contact with other infected animals, their blood, or other tissues. The envelope protein of these virus…

Nucleic-acid-amplification tests (NAATs) should be used to differentiate dengue from Zika virus infection in most patients with clinical illnesses compatible with either infection, according to a new report from the Centers for Disease Control and Prevention (CDC).Reuters Health Information

The U.S. diagnostic market celebrated three regulatory firsts last week. 
1. FDA Authorizes Marketing of First Zika Test
Until now, tests for detecting Zika virus immunoglobulin (IgM) antibodies in blood were only authorized for emergency use under FDA’s Emergency Use Authorization (EUA) authority. On Thursday, the agency said it has authorized marketing of the ZIKV Detect 2.0 IgM Capture ELISA under the agency’s traditional premarket authority. The test is manufactured by InBios International.
“At the onset of the Zika virus outbreak, when little was known about the disease or how…

Source: MDDICategory: Medical Devices Authors: Tags: Regulatory and Compliance IVD Source Type: news

We describe the unexpected binding of S-layer to cells devoid of DC-SIGN but also confirm that the presence of DC-SIGN was essential for S-layer’s antiviral activity. S-layer protein exerted its antiviral effect with different kinetics than mannan, a known viral inhibitor that also acts on DC-SIGN (Yu et al., 2017). Together our results suggest that inhibition of viral entry by S-layer occurs via a novel S-layer/DC-SIGN interaction.
Materials and Methods
Isolation of S-Layer Proteins
S-layer proteins were extracted from overnight cultures of L. acidophilus ATCC 4356 cells grown in MRS medium at 37°C by using 6…

In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given…

Experimental Analysis of Viral–Host Interactions

Joseph Gillen and Aleksandra Nita-Lazar*

Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States

Viral and pathogen protein complexity is often limited by their relatively small genomes, thus critical functions are often accomplished by complexes of host and pathogen proteins. This requirement makes the study of host–pathogen interactions critical for the understanding of pathogenicity and virology. This review article discusses proteomic methods that offer an …

A brand new edition of the popular “Environment and Health A to Z” booklet includes eye-catching illustrations and easy-to-understand text. (read more)

(PLOS) Immune cells called CD4+ T cells could be important mediators of protection against the Zika virus, according to a study published Jan. 24 in the open-access journal PLOS Pathogens by Sujan Shresta of the La Jolla Institute for Allergy and Immunology, and colleagues. The findings support vaccine strategies that induce a protective CD4+ T cell response to the Zika virus.



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