Genetic screen identifies genes that protect cells from Zika virus


(American Friends of Tel Aviv University) A new Tel Aviv University study uses a genetic screen to identify genes that protect cells from Zika viral infection. The research may one day lead to the development of a treatment for Zika and other infections.

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Publication date: Available online 16 August 2019Source: MicronAuthor(s): Lilian Mirian Oliveira de Morais, Eduardo Inocente Jussiani, João Antonio Cyrino Zequi, Paulo José dos Reis, Avacir Casanova AndrelloAbstractAedes aegypti and Aedes albopictus are vectors of several arboviruses responsible for causing dengue, chikungunya, Zika, and yellow fever viruses and microcephaly, resulting in a public health problem in several countries worldwide. Even in this scenario, studies on the morphology of mosquito eggs are still lacking. In this paper, X-ray microtomography was used to study mosquito egg morphology. Sev…

Source: MicronCategory: Biology Source Type: research

Severe birth defects caused by the Zika virus may be related to the antibodies infected mothers produce to fight the disease, a study by YSPH researchers shows.

CONCLUSIONS: The co-circulation of those viruses in the same area represents a risk for the development of severe infections if they spread throughout the country. Successive flavivirus infections may have an impact on disease pathogenesis, as well as on the development of safe vaccine strategies.
PMID: 31411310 [PubMed – in process]

Source: Memorias do Instituto Oswaldo CruzCategory: Infectious Diseases Authors: Tags: Mem Inst Oswaldo Cruz Source Type: research

Contributors : Lauretta A Lacko ; Lei Tan ; Shuibing ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo determine the effect of Zika virus infection on pre-implantation embryonic development, we performed single blastocyst RNA-Seq on MOCK and ZIKV infected embryos. ZIKV infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the mechanisms underlying fetal loss remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pregnancy, or pre- and peri-implantation, because most current studies of Z…

Source: GEO: Gene Expression OmnibusCategory: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

Zika virus (ZIKV) infection during pregnancy is associated with microcephaly, a congenital malformation resulting from neuroinflammation and direct effects of virus replication on the developing central nervous system (CNS). However, the exact changes in the affected CNS remain unknown. Here, we show by transcriptome analysis (at 48 hours post-infection) and multiplex immune profiling that human induced-neuroprogenitor stem cells (hiNPCs) respond to ZIKV infection with a strong induction of type I interferons (IFNs) and several type I IFNs stimulated genes (ISGs), notably cytokines and the pro-apoptotic chemokines CXCL9 an…

This study defines grp94 as a crucial host factor for flavivirus replication and identified CDDO-me as a potent small molecule inhibitor of flavivirus infection. Inhibition of grp94 may contribute to the antiviral activity of CDDO-me. Further investigation of grp94 inhibitors may lead to a new class of broad-spectrum anti-flaviviral medications.

Conclusions: The results showed that the network topology exhibits the features of a scale-free network instead of a random network. Focal hubs are highlighted and the critical period is found. Outcomes are important for the researchers, health officials, and policy makers who deal with arbovirus epidemic diseases. Zika virus and Chikungunya virus can also be modelled and analyzed in this manner.
PMID: 31406610 [PubMed]

Source: World Health Organization (WHO). Published: 8/14/2019.
This 25-minute video features live responses from an expert to questions about dengue, which is transmitted through Aedes aegypti and Aedes Albopictus mosquitoes, also known as Asian tiger mosquitoes. These are the same mosquitoes that transmit chikungunya, yellow fever, and Zika. Topics include identifying the warning signs and symptoms of severe dengue, and complications caused by dengue.
(Video or Multimedia)

Zika virus (ZIKV) is a newly emerging flavivirus that broadly exhibits in various bodily tissues and fluids, especially in the brain, and ZIKV infection often causes microcephaly. Previous studies have been reported that ZIKV can infect renal cells and can be detected in the urine samples of infected individuals. However, whether ZIKV infection causes renal diseases and its pathogenic mechanisms remains unknown. Here, we identified that ZIKV infection resulted in acute kidney injury (AKI) in both newborn and adult mouse models by increasing the levels of AKI-related biomarkers [e.g., serum creatinine (Scr), kidney injury m…

Conclusions/SignificanceThe two barriers associated withAe.aegypti midgut prevent YFV-17D replication. Our study contributes to our basic understanding of vector –pathogen interactions and may also aid in the development of non-transmissible live virus vaccines.

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