Spleen microbes of wild animals change with tick-borne illness

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(PLOS) Anaplasmosis, a tick-borne febrile disease, can be carried by wild mammals before being transmitted to humans through a tick bite. Now, researchers reporting in PLOS Neglected Tropical Diseases have found that Anaplasma bacteria alter the patterns of other microbes in the spleens of mice and shrews.

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Publication date: Available online 26 August 2019Source: Ticks and Tick-borne DiseasesAuthor(s): F. Boucher, Y. Moutroifi, B. Peba, M. Ali, Y. Moindjie, A.-S. Ruget, S. Abdouroihamane, Kassim A. Madi, M. Soulé, O. Charafouddine, C. Cêtre-Sossah, E. CardinaleAbstractTick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria parva, Theiler 1904, anaplasmosis …

Type IV secretion systems (T4SS) are used by a number of bacterial pathogens to attack the host cell. The complex protein structure of the T4SS is used to directly translocate effector proteins into host cells, often causing fatal diseases in humans and animals. Identification of effector proteins is the first step in understanding how they function to cause virulence and pathogenicity. Accurate prediction of effector proteins via a machine learning approach can assist in the process of their identification. The main goal of this study is to predict a set of candidate effectors for the tick-borne pathogen Anaplasma phagocy…

We report a case of a 64-year-old man presenting with a febrile illness and acute nephritis in the setting of HGA infection. The patient’s kidney biopsy was characterized by a membranoproliferative glomerulonephritis pattern and acute interstitial inflammation. After appropriate antibiotic treatment and high-dose steroids, the patient had a marked improvement in kidney function, although a subsequent recrudescence of nephritis required a 6-month course of additional steroids. As the prevalence of tick-borne diseases continues to spread across the United States, raising awareness of the potential for atypical presenta…

We report here the case of a 5-year-old cow from Germany with clinically overt granulocytic anaplasmosis presenting with fever, lower limb oedema and drop in milk-yield. The herd encompassed 10 animals, 8 other animals showed subclinical infection. The strains from the 9 A. phagocytophilum positive cows were molecularly characterized using ankA gene-based and multilocus sequence typing (MLST). Seven of 9 (78%) animals were infected simultaneously with different ankA variants belonging to ankA clusters I and IV. MLST analysis also revealed the presence of multiple strain types. This could be due to co-transmission or superi…

Authors: Figoni J, Chirouze C, Hansmann Y, Lemogne C, Hentgen V, Saunier A, Bouiller K, Gehanno JF, Rabaud C, Perrot S, Caumes E, Eldin C, de Broucker T, Jaulhac B, Roblot F, Toubiana J, Sellal F, Vuillemet F, Sordet C, Fantin B, Lina G, Gocko X, Dieudonné M, Bodaghi B, Gangneux JP, Degeilh B, Partouche H, Lenormand C, Sotto A, Raffetin A, Monsuez JJ, Michel C, Boulanger N, Cathebras P, Tattevin P, endorsed by scientific societies
Abstract
Lyme borreliosis is transmitted en France by the tick Ixodes ricinus, endemic in metropolitan France. In the absence of vaccine licensed for use in humans, primary prevent…

Publication date: Available online 15 May 2019Source: Small Ruminant ResearchAuthor(s): Adil Khan, Nasreen, Robert D. Mitchell, Sadaf Niaz, Sultan Ayaz, Irfan Khattak, Huma Naeem, Adalberto A. Pérez de León, Muhammad Arfan ZamanAbstractAnaplasmosis is a widespread tick-borne rickettsial disease responsible for fever, weakness, dyspnea, and progressive anemia in small ruminants, including sheep, goats, deer, and antelope. Anaplasma spp., the causative agent of anaplasmosis, are mainly transmitted via infected ticks to susceptible hosts during blood feeding. These pathogens are particularly devastating to sheep…



Larissa A. Martins1, Camila D. Malossi1†, Maria F. B. de M. Galletti1†, José M. Ribeiro2, André Fujita3, Eliane Esteves4, Francisco B. Costa5, Marcelo B. Labruna5, Sirlei Daffre1 and Andréa C. Fogaça1*

1Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
2Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, United States
3Departamento de Ciência da Computação, Instituto de Matemática e Estatística,…

In this study, we developed a recombinase polymerase amplification (RPA) assay to detect A. phagocytophilum in clinical samples. Following alignment of the relevant DNA sequences, a pair of specific primers based on the 16S rRNA gene was designed to specifically detect A. phagocytophilum. The assay was performed at a constant temperature of 38 °C for 30 min, with a final primer concentration of 0.4 μM. The specificity of the primers was confirmed when DNA from A. phagocytophilum was used as the positive control, and DNA from other related pathogens were used as the negative controls, with ddH2O acting as the b…

Source: Experimental ParasitologyCategory: Parasitology Authors: Tags: Exp Parasitol Source Type: research

In this study, we applied complementary in silico approaches to modeling how Anaplasma phagocytophilum infection modulates tick vector regulome. This proof-of-concept research provided support for the use of network analysis in the study of regulome response to infection, resulting in new information on tick-pathogen interactions and potential targets for developing interventions for the control of tick infestations and pathogen transmission. Deciphering the precise nature of circuits that shape the tick regulome in response to pathogen infection is an area of research that in the future will advance our knowledge of tick-…

Publication date: Available online 6 April 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Jeremy W. Turck, Vikas Taank, Girish Neelakanta, Hameeda SultanaAbstractAnaplasma phagocytophilum, the agent of human anaplasmosis, is an obligate intracellular bacterium that uses multiple survival strategies to persist in Ixodes scapularis ticks. Our previous study showed that A. phagocytophilum efficiently induced the tyrosine phosphorylation of several Ixodes proteins that includes extended phosphorylation of actin at tyrosine residue Y178. In order to identify the tyrosine kinase responsible for the A. phagocytophilum induce…



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